Comprehensive transcriptomic and proteomic analyses identify intracellular targets for myriocin to induce Fusarium oxysporum f. sp. niveum cell death
نویسندگان
چکیده
Abstract Background Myriocin is a natural product with antifungal activity and derived from Bacillus amyloliquefaciens LZN01. Our previous work demonstrated that myriocin can inhibit the growth of Fusarium oxysporum f. sp. niveum (Fon) by inducing membrane damage. In this study, actions against Fon were investigated focus on effects intracellular molecules. Results Analysis DNA binding fluorescence spectra interact dsDNA cells. The intracellular-targeted mechanism action was also supported transcriptomic proteomic analyses; total 2238 common differentially expressed genes (DEGs) identified. DEGs further verified RT-qPCR. Most assigned metabolism genetic information processing functions enriched in ribosome biogenesis eukaryotes pathway. expression some proteins pathway affected myriocin, primarily controlled C6 zinc cluster transcription factor family NFYA factor. influenced posttranscriptional gene products triggering main RI (retained intron) events novel alternative splicing; targeted key ( FOXG_09470 ) or (RIOK2) pathway, resulting disordered translation. Conclusions conclusion, determined to exhibit targeting results our study may help elucidate Fon.
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ژورنال
عنوان ژورنال: Microbial Cell Factories
سال: 2021
ISSN: ['1475-2859']
DOI: https://doi.org/10.1186/s12934-021-01560-z